Anti-adipogenic effects of Maclura tricuspidata twig extract in 3T3-L1 pre-adipocytes

This study was conducted to evaluate the anti-adipogenic effects of Maclura tricuspidate twig extract (MTE) in 3T3-L1 pre-adipocytes. pre-adipocytes were differentiated with adipogenic regents (dexamethsone, 3-isobutyl-methylxanthine, insulin) by incubation for 8 days absence or presence MTE. The accumulation lipid droplets and triglycerides cells dose-dependently suppressed MTE caused an increase release adiponectin inhibited leptin. Consistent these results, attenuated protein expression peroxisome proliferator-activated receptor gamma (PPARγ), CCATT/enhancer binding alpha (C/EBPα), fatty acid synthase (FAS) adipocyte Protein 2 (aP2), all which are associated adipogenesis cells. Treatment also increased free glycerol. Additionally, hormone-sensitive lipase (HSL) adipose triglyceride (ATGL), lipolysis. These results showed that inhibits regulating proteins involved adipogenesis. Therefore, suggest has potential be used as a functional ingredient food industry prevent obesity.